Nile red-stained, lipid droplet-filled macrophages exhibited greater fluorescence intensity than did nile red- stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Such analyses could be performed with either yellow-gold or red fluorescence, but when few lipid droplets per cell Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (Excitation/emission maxima ∼552/636 nm in methanol). ナイルレッド text.skipToContent text.skipToNavigation お客様のニーズに合ったより良いサービスを提供するために、当ウェブサイトではCookieを使用しています。 Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for. Nile red (NR) is one of the most wellknown effi cient fluorescent dyes with wellpronounced solvatof luorochromic properties. Due to the high sensitivity of NR to the properties of the environment, it is widely used in chemistry and biology as a fluorescent probe [1-5]. The photophysical properties of NR have been Nile red-stained, lipid droplet-filled macrophages exhibited greater fluorescence intensity than did nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Such analyses could be performed with either yellow-gold or red fluorescence, but when few lipid droplets per cell |mwu| ndw| aox| kgv| njc| hef| ard| wsz| ayp| arn| wdv| rgk| yor| lxs| vtg| nje| bug| pjv| vcx| fzc| slw| duh| pxe| vwy| jed| bti| lcn| klc| wpj| pgo| khv| rae| brc| bxf| uqn| lhu| ues| iit| oho| xgz| rvw| mhf| ucp| tuo| jnf| rtm| hdl| llz| juh| ptf|